In silico comparative genomics analysis of Plasmodium falciparum for the identification of putative essential genes and therapeutic candidates.

A sequence of computational methods was used for predicting novel drug targets against drug resistant malaria parasite Plasmodium falciparum. Comparative genomics, orthologous protein analysis among same and other malaria parasites and protein-protein interaction study provide us new insights into determining the essential genes and novel therapeutic candidates. Among the predicted list of 21 essential proteins from unique pathways, 11 proteins were prioritized as anti-malarial drug targets. As a case study, we built homology models of two uncharacterized proteins using MODELLER v9.13 software from possible templates. Functional annotation of these proteins was done by the InterPro databases and from ProBiS server by comparison of predicted binding site residues. The model has been subjected to in silico docking study with screened potent lead compounds from the ZINC database by Dock Blaster software using AutoDock 4. Results from this study facilitate the selection of proteins and putative inhibitors for entry into drug design production pipelines

Synthesis, Structure, and Antimalarial Activity of Tricyclic 1,2,4-Trioxanes Related to Artemisinin

Two sets of tricyclic 1,2,4-trioxanes containing the ABC (10, 11) and ACD ring portions (21, 22, 32, 33, 37, and 38) of artemisinin (1) were synthesized by successive photo-oxygenation of appropriate enol-ether precursors to 1,2-dioxanes and inter- and intramolecular reaction with a carbonyl compound or oxo-substituted side-chain. The structures of 10, 21, and 22 were determined by X-ray analysis. The anti-malarial activity of all trioxanes, except 37 and 38, was evaluated in vitro against chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum parasites. Trioxanes 11 and 21 were as active as artemisinin (1). It was found that neither the lactone function nor rings B and D of 1 were essential for activity. A possible pharmacophore for artemisinin-like activity, which embodies a spirocyclopentane group attached to C(3) of 1,2,4-trioxane, was proposed

SIMPLIFIED DIAGNOSIS OF MALARIA INFECTION: GFM/PCR/ELISA A SIMPLIFIED NUCLEIC ACID AMPLIFICATION TECHNIQUE BY PCR/ELISA

We report an adaptation of a technique for the blood sample collection (GFM) as well as for the extraction and amplification of Plasmodium DNA for the diagnosis of malaria infection by the PCR/ELISA. The method of blood sample collection requires less expertise and saves both time and money, thus reducing the cost by more than half. The material is also suitable for genetic analysis in either fresh or stored specimens prepared by this method.<br>Relatamos a adaptação de uma técnica para coleta de amostras (MFV) e outra para extração, amplificação de DNA de parasitas da malária para diagnóstico por PCR/ELISA. O método de coleta de amostras requer menos habilidade e economisa tempo e dinheiro, assim reduzindo a mais da metade o custo. O material é também adequado para análise genética em especimens frescos ou estocados, preparados por este método

Bench Aids for the diagnosis of intestinal parasites

A set of nine A-4 colour plates with 118 photomicrographs illustrating the appearance and diagnostic features of all the common intestinal helminths and protozoan parasites known to infect humans. Produced in a robust plasticized format, the plates can be used as either a guide for laboratory and field workers in endemic countries or a teaching aid for students and trainees. The aim is to help the microscopist ascertain the presence of parasites in faeces, whether they be minute protozoan cysts or large helminth eggs, and to identify them correctly. With this goal in mind, the bench aids include pertinent laboratory instructions as well as high-quality images. The photomicrographs illustrate diagnostic features of each of the parasites as they appear in different preparations and at different magnifications. Each photomicrograph is produced with a measuring bar and accompanied by a short explanatory legend, which draws attention to distinctive features that help confirm diagnosis. Helminth eggs are illustrated in the first 36 photomicrographs, which show the diagnostic stages of the most common helminths, including nematodes, cestodes, schistosomes, and other trematodes. The remaining photomicrographs offer advice on the more difficult task of detecting and identifying intestinal protozoan trophozoites and cysts. Relevant laboratory techniques are described on the reverse side of the plates. Additional laboratory aids include dichotomous keys for the identification of amoebic trophozoites, trophozoites of intestinal flagellates, and cysts of amoebae and flagellate

Gênero e insegurança no trabalho no Reino Unido Gender and employment insecurity in the UK

O objetivo deste artigo é explorar a dimensão de gênero da insegurança do emprego no Reino Unido, focalizando, para tanto, as recentes tendências do trabalho temporário, que incluem o de prazo fixo, o sazonal, o sem vínculo empregatício, o contratado através de agência, o ocasional e outros tipos de trabalho temporário. Essas formas de trabalho são, inequivocamente, inseguras, sejam elas livremente escolhidas ou não. A primeira seção do artigo trata das pressões e resistências que têm, cumulativamente, levado à atual participação de mulheres no trabalho remunerado. Examinam-se, em seguida, as recentes tendências nos padrões de emprego no Reino Unido, com especial referência às diferenças entre mulheres em idade de trabalho, bem como à incidência e distribuição do trabalho de meio período. As tendências recentes e as diferenças de gênero no trabalho temporário são, assim, consideradas à luz desse contexto mais amplo. Finalmente, esses achados são confrontados com os argumentos no sentido de que o emprego atípico fornece oportunidades para um aumento da participação de mulheres na força de trabalho e para uma conciliação prática entre o emprego e os papéis e relacionamentos familiares.<br>The objective of this article is to explore how far employment insecurity is gendered in the UK, focusing on recent trends in temporary employment, covering fixed term, seasonal, agency, casual and other temporary work. The first section of this paper considers the pressures and resistances that have cumulatively led to women's current participation in paid work. This is followed by an examination of recent trends in UK employment patterns, with particular reference to differences within the female population of working age and the incidence and distribution of part-time employment. Recent trends and gender differences in temporary employment are then considered against this broader background. Finally, this evidence is considered in relation to arguments that atypical employment provides opportunities for women's increased labour force participation and the practical reconciliation of employment and family roles and relationships